{"type":"rich","version":"1.0","provider_name":"Transistor","provider_url":"https://transistor.fm","author_name":"Vector Signals","title":"Bunyavirus NSm: Key to Transmission Between Vertebrates and Mosquitoes (January 2025)","html":"<iframe width=\"100%\" height=\"180\" frameborder=\"no\" scrolling=\"no\" seamless src=\"https://share.transistor.fm/e/7c561d73\"></iframe>","width":"100%","height":180,"duration":1167,"description":"Briefing Document: NSm Protein's Critical Role in Bunyavirus Transmission\nCitation: Terhzaz, S., Kerrigan, D., Almire, F. et al. NSm is a critical determinant for bunyavirus transmission between vertebrate and mosquito hosts. Nat Commun 16, 1214 (2025). https://doi.org/10.1038/s41467-024-54809-7Date: Received - 03 May 2024 | Accepted - 21 November 2024 | Published - 31 January 2025Prepared for: Saleh Lab\nKey Themes:NSm as a Unique Feature of Vector-Borne Bunyaviruses: The study establishes a strong correlation between the presence of the non-structural protein NSm and the ability of bunyaviruses to be transmitted by arthropod vectors (arboviruses). Non-arboviral bunyaviruses do not encode NSm.NSm's Dispensability in Monolayer Cell Cultures: While essential for in vivo transmission, NSm is shown to be largely dispensable for viral replication in both mammalian and mosquito cell lines in vitro.NSm's Crucial Role in Mosquito Midgut Infection and Dissemination: The research demonstrates that NSm is absolutely required for Bunyamwera virus (BUNV) to successfully infect the female Aedes aegypti mosquito following a blood meal. Specifically, it is critical for cell-to-cell spread and egress from the mosquito midgut, a major barrier to viral infection.Midgut-Specific Requirement: The necessity of NSm is specific to the midgut. When this barrier is bypassed through intrathoracic injection, the NSm deletion mutant (BUN-ΔNSm) can effectively infect other mosquito tissues, including the salivary glands, and be transmitted in saliva.NSm Facilitates Infection Foci Formation: In the midgut, BUNV-wt infection leads to the formation of large clusters of infected cells (infection foci), while BUN-ΔNSm remains confined to initially infected cells and fails to spread.NSm Acts Early and Transiently at the Periphery of Infection Foci: Immunocytochemistry reveals that NSm protein is localized mainly at the periphery of infection foci in the midgut, suggesting an early and transient...","thumbnail_url":"https://img.transistorcdn.com/qJYlR2Phxe3IMx6KHnsmKp1D71DIqj8LuYMSrDKo9Jc/rs:fill:0:0:1/w:400/h:400/q:60/mb:500000/aHR0cHM6Ly9pbWct/dXBsb2FkLXByb2R1/Y3Rpb24udHJhbnNp/c3Rvci5mbS82MDgx/MDIyNmJkNWU5YmIz/NzJhZDVmZjYyOGZi/NTgxMi53ZWJw.webp","thumbnail_width":300,"thumbnail_height":300}