{"type":"rich","version":"1.0","provider_name":"Transistor","provider_url":"https://transistor.fm","author_name":"Protein Production Technology Podcast","title":"Holger Mueller, BlueSens | Using Off-Gas Analysis to Improve Fermentation and Scale-Up: What can the gases coming out of a bioreactor reveal about the cells inside it?","html":"<iframe width=\"100%\" height=\"180\" frameborder=\"no\" scrolling=\"no\" seamless src=\"https://share.transistor.fm/e/9c06edd7\"></iframe>","width":"100%","height":180,"duration":1630,"description":"Powered by Protein Production Technology International\nWhat can the gases coming out of a bioreactor reveal about the cells inside it? And how can that information help producers improve yields, control feeding, and scale fermentation processes more reliably?\nIn this episode of the Protein Production Technology International Podcast, Nick Bradley speaks with Holger Mueller, CEO of BlueSens Smart Bioprocess Solutions, about how off-gas analysis can provide deeper insight into cell metabolism and help operators make better decisions throughout fermentation.\nHolger explains how respiratory quotient (RQ), carbon dioxide emission rate (CER), and oxygen uptake rate (OUR) can reveal metabolic shifts, substrate depletion, and oxygen limitations. He explores how RQ-based feeding strategies can help prevent overfeeding and underfeeding, reduce unwanted byproducts, and improve process performance compared with conventional feeding profiles.\nThe conversation also examines the role of process analytical technology (PAT) in scale-up, including how respiratory data can help identify differences in cellular behavior between laboratory and production-scale bioreactors. Holger discusses the importance of collecting meaningful data early in development, translating measurements into biological insights, and maintaining consistent, reliable analysis across different scales.\nFinally, Holger shares how BlueSens’ gas analysis technology, including its Blueberry system, is designed to support fermentation monitoring from R&D through commercial production — and why researchers should make better use of the data they are already collecting.\nThe Future of Protein Production is connecting companies working to make the global protein system more sustainable, kind and fair for the planet, people, and animals.\nTo find out more about our platform click here: https://www.futureofproteinproduction.com/\nTo read the most recent edition of Protein Production Technology, our multi-media magazine,...","thumbnail_url":"https://img.transistorcdn.com/tUifNxYi7y1wl92YvdM05mMBe8G47RO6Jcub5_A_xxY/rs:fill:0:0:1/w:400/h:400/q:60/mb:500000/aHR0cHM6Ly9pbWct/dXBsb2FkLXByb2R1/Y3Rpb24udHJhbnNp/c3Rvci5mbS9zaG93/LzM5MjkxLzE2OTk5/NTU4MzQtYXJ0d29y/ay5qcGc.webp","thumbnail_width":300,"thumbnail_height":300}